核磁共振研究室

TEL: +886-2-28712121 Ext.2703 E-mail: thlin@vghtpe.gov.tw
Principal Investigator: Ta-Hsien Lin
1984	B.S., Dept. Chem., Natl. Taiwan Normal Univ.
1993	Ph.D., Dept. Chem., Univ. Calif., San Diego, USA
1993 ~ 1996	Postdoc. Fellow, Inst. Biomed., Academia Sinica
1996 ~ 2000	Assoc. Investigator, Dept. Med. Res. & Edu., Veterans General Hospital-Taipei
1998 ~ 2000	Adjunct Assoc. Prof., Grad. Inst. Biochem., Natl. Yang-Ming Univ.
2000~ present	Adjunct Assoc. Investigator, Dept. Med. Res. & Edu., Veterans General Hospital-Taipei
2000~ 2007	Assoc. Prof., Grad. Inst. Biochem., Natl. Yang-Ming Univ.
2007 ~ present	Prof., Grad. Inst. Biochem., Natl. Yang-Ming Univ.
2007 ~ present	Prof., Grad. Inst. Biomedical Informatics., Natl. Yang-Ming Univ.
2007 ~ present	Prof., Grad. Inst. Biochemistry and Molecular Biology., Natl. Yang-Ming Univ
2007 ~ present	Prof., Department of Life Sciences & Grad. Inst. Genome Sciences., Natl. Yang-Ming Univ.


Technologist:	Fang-Min Lin
PhD Students:	Yi-Choang Huang, Yi-Ru Chen, Chi- Jen Lo, Chih-Ching Wang
Master Student:	Jun-Lin Cheng, Alven Chung, Liang-Chun Lin, Yi-Shiuan Tsai, Chu-Ting Liang

<Research Interests>
Structural biology study may provide detailed molecular and atomic level structural information of biomolecules to correlate with their biological functions in physiological states and disturbances in disease states.
In our disease-oriented study, we proposed to em- ploy high resolution heteronuclear multi-dimensional NMR spectroscopy to determine the structure of human apolipoprotein E (apoE) and b-amyloid peptide (Ab) which are two key biomacromolecules in the patho- genesis of atherosclerosis and Alzheimer's disease.
Structural determination of protein phosphatase inhibitor-2 was also studied by NMR spectroscopy as to reveal the molecular mechanism of phosphorylation and activation to some extent from the structural point of view .
Another project was also performed to develop NMR spectroscopy as a new technology for the analysis of lipids and lipoproteins with atherosclerosis indication.

<Selected Recent Publications>
1. Hsien-bin Huang*, Yi-Chen Chen, Ting-Ting Lee, Yi-Choang Huang, Hsin-Tzu Liu, Chen-Kuang Liu, Huey-Jen Tsay and Ta-Hsien Lin* (2007), "Structural and Biochemical Characterization of Inhibtor-1a", PROTEINS: Structure, Function and Bioinformatics, 68, 779-788.

2. M.Q. Liao, Y. J. Tzeng, Lea. Y. X. Chang, H. B. Huang, T. H. Lin, C. L. Chyan, Y. C. Chen (2007), "The correlation between neurotoxicity, aggregative ability and secondary structure studied by sequence truncated Ab peptides", FEBS Lett., 581, 1161-1165.

3. Shu-Chen Kao, Chun-Yu Chen, Shiu-Lan Wang, Jaw-Ji Yang, Wei-Chuan Hung, Yi-Chen Chen, Ning-Sheng Lai, Hsin-Tzu Liu, Hsien-lu Huang, Hong-Chen Chen, Ta-Hsien Lin*, Hsien-bin Huang* (2007), "Identification of phostensin, a PP1 F-actin cytoskeleton targeting subunit", Biochem. Biophy. Res. Commun., 356, 594-598.

4. Y. R. Chen, H. B. Huang, C. L. Chyan, M. S. Shiao, T. H. Lin* and Y. C. Chen* (2006), "The Effect of Ab Conformation on the Metal Affinity and Aggregation Mechanism Studied by Circular Dichroism Spectroscopy", J. Biochem., 139, 733-740.

5. Ta-Hsien Lin, Pai-Chi Tsai, Hsin-Tzu Liu, Yi-Chen Chen, Lan-Hsin Wang, Fu-Kai Hsieh and Hsien-bin Huang (2005), "Characterization of the Protein Phosphatase 1–binding Motifs of Inhibitor-2 and DARPP-32 by Surface Plasmon Resonance", J. Biochem., 138, 697-700.

6. Chia-Lin Chyan, Po-Chung Huang, Ta-Hsien Lin, Jian-Wen Huang, S. S. Lin, Hsien-bin Huang and Yi-Chen Chen (2005), "Purification, crystallization and preliminary crystallographic studies of a Camodulin-OLFp molecule", Acta Cryst., F61, 785-787.

7. Lou, B.-S., Lin, T.-H and Lo. C.-Z (2005), "The interactions of phenytoin and its binding site in DI-S6 segment of Na+ channel voltage-gated peptide by NMR spectroscopy and molecular modeling study", J. Peptide Res., 66, 27-38.

8. Yu Ho, Jye-Chian Hsiao, Min-Hsiang Yang, Che-Sheng Chung, Yu-Chang Peng, Ta-Hsien Lin, Wen Chang and Der-Lii M. Tzou (2005), "The Oligomeric Structure of Vaccinia Viral Envelope Protein A27L is Essential for Binding to Heparin and Heparan Sulfates on Cell Surfaces: A Structural and Functional Approach Using Site-specific Mutagenesis", J. Mol. Biol., 349, 1060-1071.

9. Ta-Hsien Lin, Hsien-bin Huang, H.-A. Wei, S.-H. Shiao and Y. C. Chen (2005), "The effect of temperature and lipid on the conformational transition of gramicidin A in lipid vesicles", Biopolymers, 78, 179-186.

10. Chi-Yuan Chou, Yi-Ling Lin, Yu-Chyi Huang, Sheh-Yi Sheu, Ta-Hsien Lin, Huey-Jen Tsay, Gu-Gang Chang and Ming-Shi Shiao (2005), "Structural Variation in Human Apolipoprotein E3 and E4: Secondary Structure, Tertiary Structure, and Size Distribution", Biophys. J., 88, 455-466.

11. Sergiy I. Tyukhtenko, Yao-Te Huang, Ta-Hsien Lin, Chinpan Chen, Chi-Fon Chang, Shin-Jye Lee, Alexandra V. Litvinchuka, Jei-Fu Shaw, Yen-Chywan Liaw and Tai-huang Huang (2004), "Probing the Enzyme Catalytic Mechanism by Nuclear Magnetic Resonance - A Case Study of a Serine Protease", J. Chin. Chem. Soc., 51, 1133-1140.

12. Ta-Hsien Lin, Jian-Wen Huang, Hsien-bin Huang, Yi-chen Chen, Chia-Yen Liu, Chi-jen Lo, Tzu Chun Tang and Chia-Lin Chyan,(2004), "Letter to the Editor: 1H, 15N, and 13C resonance assignments of calmodulin complexed with the calmodulin-binding domain of calcineurin", J. Biomol. NMR, 29, 531-532.

13. Lou, B.-S., Lin, T.-H and Lo. C.-Z (2004), "The conformation and movement of Na+ channel inactivation gate peptide in linker between domain III and IV during inactivation by NMR spectroscopy and molecular modeling study", J. Peptide Res., 63, 313-323.

14. Ta-Hsien Lin, Yi-Choang Huang, Mei-ling Chin, Yi-chen Chen, Hao-Hsuan Jeng, Fang-Min Lin, Ming-Shi Shiao, Atsuko Horiuchi, Paul Greengard, Angus C. Nairn and Hsien-bin Huang (2004), "Letter to the Editor: 1H, 15N, and 13C resonance Assignments of DARPP-32 (dopamine and cAMP-regulated phosphoprotein, Mr. 32,000) – a Protein Inhibitor of Protein Phosphatase-1", J. Biomol. NMR, 28, 413-414.

15. Ta-Hsien Lin, Yi-Chen Chen, Chia-lin Chyan, Li-huang Tsay, Tzu Chun Tang, Hao-Hsuan Jeng, Fang-Min Lin and Hsien-bin Huang (2003), "Phosphorylation by glycogen synthase kinase of inhibitor-2 does not change its structure in free state", FEBS Lett., 554, 253-256.

16. Ta-Hsien Lin, Chih-Ming Chia, Jye-Chian Hsiao, Wen Chang, Chiao-Chu Ku, Shang-Cheng Hung and Der-Li M. Tzou (2002), "Structural Analysis of the Extracellular Domain of Vaccinia Virus Envelope Protein, A27L, by NMR and CD Spectroscopy", J. Biol. Chem., 277, 20949-20959.

17. Hsin-Tzu Liu, Ta-Hsien Lin, Hann-Chrong Kuo, Yi-Chen Chen, Huey-Jen Tsay, Hao-Husan Jeng, Pai-Chi Tsai, Fu-Kai Shie, Ji-Hsiung Chen and Hsien-bin Huang (2002), "Identification of the Alternative Splice Products Encoded by the Human Protein Phosphatase Inhibitor-1 Gene", Biochem. Biophy. Res. Commun, 291, 1293-1296.

18. Chia-lin Chyan, Tzu Chun Tang, Yi-chen Chen, Hsin-tzu Liu, Fang-Min Lin, Chen-Kung Liu, Meng-Juei Hsieh, Ming-Shi Shiao, Hsien-bin Huang and Ta-Hsien Lin* (2001) "Backbone 1H, 15N, and 13C Resonance Assignments of Inhibitor-1 – a Protein Inhibitor of Protein Phosphatase-1", J. Biomol. NMR, 21, 287-288.

19. Hsien-bin Huang, Yi-chen Chen, Atsuko Horiuchi, Li-huang Tsai, Hsin-tzu Liu, Chia-lin Chyan, Meng-Juei Hsieh, Chen-Kung Liu, Fang-Min Lin, Paul Greengard, Angus C. Nairn, Ming-Shi Shiao and Ta-Hsien Lin* (2001) "Backbone 1H, 15N, and 13C resonance assignments of ARPP-19". J. Biomol. NMR, 19, 383-384.

20. Hsien-bin Huang, Yi-chen Chen, Li-huang Tsai, Hui-chun Wang, Fang-Min Lin, Atsuko Horiuchi, Paul Greengard, Angus C. Nairn, Ming-Shi Shiao and Ta-Hsien Lin* (2000), "Backbone 1H, 15N, and 13C resonance assignments of inhibitor-2 - a protein inhibitor of protein phosphatase-1", J. Biomol. NMR, 17, 359-360.